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KMID : 0545119910010010012
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Journal of Microbiology and Biotechnology
1991 Volume.1 No. 1 p.12 ~ p.16
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Characterization of lac^+ gal^+ Strains of Zymomonas mobilis for Ethanol Production from Lactose
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CHO, DONG-WUK
DELANEY, STEPHEN F./ROGERS, PETER L.
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Abstract
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Previously RP1::Tn951 which is a derivative of RP1 containing the lactose transposon Tn951 was introduced into Z. mobilis strain ZM6100, and RP1::Tn951 was integrated into its genome to yield ZM6306. The galactose operon was incorporated into ZM6306 to yield ZM6307 for more efficient utilization of lactose. Batch culture study has been carried out on Z. mobilis strains, ZM6306 (lac^+) and ZM6307 (lac^+, gal^+), which can convert lactose directly to ethanol. Using a medium containing 80g·ℓ^-1 glucose and 40g·ℓ^-1 lactose, it was found that ZM6306 and ZM6307 produced maximum ethanol concentration of 40g·ℓ^-1 and 42g·ℓ^-1,respectively, whereas parent strain ZM6 produced 37g·ℓ^-1.
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